Journal of
Diabetes and Endocrinology

  • Abbreviation: J. Diabetes Endocrinol.
  • Language: English
  • ISSN: 2141-2685
  • DOI: 10.5897/JDE
  • Start Year: 2010
  • Published Articles: 59

Full Length Research Paper

Elevation of oxidative stress markers in Type 1 diabetic children

Amina Boudghene Stambouli-Guerriche
  • Amina Boudghene Stambouli-Guerriche
  • Laboratory of Physiology and Biochemistry of Nutrition, Department of Biology, University of Tlemcen, Algeria.
  • Google Scholar
Nassima Mokhtari-Soulimane*
  • Nassima Mokhtari-Soulimane*
  • Laboratory of Physiology and Biochemistry of Nutrition, Department of Biology, University of Tlemcen, Algeria.
  • Google Scholar
Hafida Merzouk
  • Hafida Merzouk
  • Laboratory of Physiology and Biochemistry of Nutrition, Department of Biology, University of Tlemcen, Algeria.
  • Google Scholar
Sid-Ahmed Merzouk
  • Sid-Ahmed Merzouk
  • Department of Technical Sciences, Faculty of Engineering, University of Tlemcen, Algeria.
  • Google Scholar
Ahmed Salih Bendedouche
  • Ahmed Salih Bendedouche
  • Pediatrics Department, University-Hospital Centre, Tlemcen, Algeria.
  • Google Scholar


  •  Received: 25 November 2014
  •  Accepted: 21 January 2015
  •  Published: 28 February 2015

Abstract

Children’s diabetes is represented by the Type 1 diabetes mellitus (T1DM). In T1DM, the persistence of hyperglycemia has been reported to cause increased production of oxygen free radicals through glucose autooxidation and nonenzymatic glycation. The aim of this study was to evaluate markers of oxidant/antioxidant status in diabetic children of Western Algeria. This study included 40 children with T1DM with mean age of 7.5 ± 1.7 years and 40 healthy age and sex matched controls. They were subjected to assessment of indicative parameters of lipoperoxidation, protein oxidation, changes in the status of antioxidant defense systems, plasma oxygen radical absorbance capacity (ORAC), glycated hemoglobin (HbA1c), total cholesterol and triglycerides. Malondialdehyde (MDA) and carbonyl proteins levels in plasma were significantly higher (4.03 ± 0.39 versus 2.53 ± 0.4 µmol/L, 5.03 ± 0.57 versus 3 ± 0.38 nmol/mg protein, respectively; P < 0.001) and a significant reduction in plasma total antioxidant capacity and vitamin C was observed in diabetic children than the controls (1.55 ± 0.28 versus 2.5 ± 0.23 AU, 37.58 ± 5.76 versus 48.8 ± 4.47 µmol/L, respectively; P < 0.001). Erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities were significantly higher (520 ± 40.42 versus 392.7 ± 42.66 U/g hemoglobin, 71.08 ± 5.18 versus 56.6 ± 2.84 U/g hemoglobin, respectively; P < 0.001), whereas erythrocyte glutathione reductase (GSH) reduced significantly (34.98 ± 2.34 versus 42.68 ± 3.03 U/g hemoglobin, respectively; P < 0.001) in diabetic children than the control subject. The present finding suggested that young diabetic patients were susceptible to oxidative stress. Appropriate support for enhancing antioxidant supply in these patients may help prevent complications due to oxidative injury. 

 

Key words: Children, oxidative stress, Type 1 diabetes mellitus.