The objectives of this research were to identify the allelic frequency of growth hormone MspI enzyme-restriction, to define the genetic inequilibrium in selected superior and inferior body weight groups of the cows (G0) and to upgrade genetically the genetic equilibrium of their Ongole-crossbred progenies (G1) population. A total of 74 blood samples were collected from G0 and G1 at the artificial insemination (AI) service center in North Sulawesi Province, Indonesia. Blood samples were screened for the presence of GH Msp1 enzyme-restriction using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method on 1.2% agarose gel. Data were analyzed using statistical program in Excel XP. The results showed that Msp1+ allele frequencies in superior and inferior G0 groups were 0.45 and 0.18, respectively. The Msp1+ allele frequencies of G1 born by superior and inferior G0 groups were 0.50 and 0.21, respectively. These increasing frequencies were contributed byMsp1+ allele inheritance from Msp1+/+ bull genotype (Krista). Allele frequencies of Msp1+ in both groups of G0 were not under genetic equilibrium. However, allele frequencies of Msp1+ in G1 population were under genetic equilibrium. The AI technique should be maintained involving various bull genotypes for breeding purpose of favorable Msp1+/- heterozygous genotypes and balancing the genetic equilibrium in progeny population.
Key words: Growth hormone Msp1 allele frequency, Ongole-crossbred cow population.
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