Journal of
Evolutionary Biology Research

  • Abbreviation: J. Evol. Biol. Res.
  • Language: English
  • ISSN: 2141-6583
  • DOI: 10.5897/JEBR
  • Start Year: 2009
  • Published Articles: 32

Full Length Research Paper

The allele frequencies of growth hormone gene on the parental and progeny of Ongole-crossbreed cattle population in the North Sulawesi of Indonesia using PCR-RFLP

Umar Paputungan1,2*, Luqman Hakim3, Gatot Ciptadi3 and Hapry F. N. Lapian1
1Department of Animal Production, Faculty of Animal Science, Sam Ratulangi University, Manado 95115, Indonesia. 2Department of Animal Production, Graduate Program, Brawijaya University, Malang 65145, Indonesia. 3Department of Animal Production, Faculty of Animal Husbandry, Brawijaya University, Malang 65145, Indonesia.
Email: [email protected]

  •  Accepted: 07 November 2012
  •  Published: 31 December 2012


The objectives of this research were to identify the allelic frequency of growth hormone MspI enzyme-restriction, to define the genetic inequilibrium in selected superior and inferior body weight groups of the cows (G0) and to upgrade genetically the genetic equilibrium of their Ongole-crossbred progenies (G1) population. A total of 74 blood samples were collected from G0 and G1 at the artificial insemination (AI) service center in North Sulawesi Province, Indonesia. Blood samples were screened for the presence of GH Msp1 enzyme-restriction using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method on 1.2% agarose gel. Data were analyzed using statistical program in Excel XP. The results showed that Msp1+ allele frequencies in superior and inferior G0 groups were 0.45 and 0.18, respectively. The Msp1+ allele frequencies of G1 born by superior and inferior G0 groups were 0.50 and 0.21, respectively. These increasing frequencies were contributed byMsp1+ allele inheritance from Msp1+/+ bull genotype (Krista). Allele frequencies of Msp1+ in both groups of G0 were not under genetic equilibrium. However, allele frequencies of Msp1+ in G1 population were under genetic equilibrium. The AI technique should be maintained involving various bull genotypes for breeding purpose of favorable Msp1+/- heterozygous genotypes and balancing the genetic equilibrium in progeny population.

Key words: Growth hormone Msp1 allele frequency, Ongole-crossbred cow population.