Journal of
Evolutionary Biology Research

  • Abbreviation: J. Evol. Biol. Res.
  • Language: English
  • ISSN: 2141-6583
  • DOI: 10.5897/JEBR
  • Start Year: 2009
  • Published Articles: 32

Full Length Research Paper

Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444)

Alfred O. Ubalua*
  • Alfred O. Ubalua*
  • Plant Tissue Culture Laboratory, Biotechnology Research and Development Center, National Root Crops Research Institute (NRCRI), Umudike, PMB 7006 Umuahia, Abia State, Nigeria.
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Ena Mbanaso
  • Ena Mbanaso
  • Iltab Laboratory, Donald Danforth Plant Science Center, 975 N, Warson Road, St. Louis, Mo 63132, USA.
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  •  Received: 23 April 2013
  •  Accepted: 07 October 2014
  •  Published: 30 October 2014


The embryogenic ability of two cassava cultivars (Sandpaper and TMS 60444) was investigated by culturing their young leaf lobes on an induction medium (DKW2 50P). The explants formed organized embryogenic structures regardless of the concentration of picloram in the medium. Organized embryogenic structures induction was enhanced by increasing the concentration of picloram in the induction medium for both cultivars. The optimum level of picloram at which maximum stable frequencies (53.1±17.6 and 51.5±14.6 for Sandpaper and TMS 60444 respectively) of organized embryogenic structures were obtained was 5 mg/l. Friable embryogenic callus were further produced in GD2 50P medium supplemented with 500 µM tyrosine. The produced friable embryogenic calluses are prime target tissues for genetic transformation and plantlet regeneration.


Key words: Cassava cultivars, explants, organized embryogenic structures, friable embryogenic callus, picloram, tyrosine.