Journal of
Environmental Chemistry and Ecotoxicology

  • Abbreviation: J. Environ. Chem. Ecotoxicol.
  • Language: English
  • ISSN: 2141-226X
  • DOI: 10.5897/JECE
  • Start Year: 2009
  • Published Articles: 197

Full Length Research Paper

Algal density assessed by spectrophotometry: A calibration curve for the unicellular algae Pseudokirchneriella subcapitata

Lúcia Helena Ribeiro Rodrigues
  • Lúcia Helena Ribeiro Rodrigues
  • Laboratório de Ecotecnologia e Limnologia, Instituto de Pesquisas Hidráulicas, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970, Porto Alegre, RS, Brazil
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Alexandre Arenzon
  • Alexandre Arenzon
  • Laboratório de Ecotoxicologia, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970, Porto Alegre, RS, Brazil.
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Maria Teresa Raya-Rodriguez
  • Maria Teresa Raya-Rodriguez
  • Laboratório de Ecotoxicologia, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970, Porto Alegre, RS, Brazil.
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Nelson Ferreira Fontoura
  • Nelson Ferreira Fontoura
  • Laboratório de Ecologia Aquática, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Av. Ipiranga 6681, 90619-900, Porto Alegre, RS, Brazil.
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  •  Accepted: 30 June 2011
  •  Published: 31 August 2011

Abstract

 

The unicellular algae Pseudokirchneriella subcapitata (Korschikov) Hindák has been frequently used for ecotoxicological tests. In this paper, a calibration curve is proposed correlating absorbance values (684 nm) and cell density for routine ecotoxicological experiments. Density (cells/mL) could be estimated as follow: Cell Density = e {[ln (absorbance_684) +16.439]/1.0219} (n=130; r2=0.9998). Residual distribution revealed that the equation could be applied for algal densities under 5,000,000 cells/mL and/or absorbance values as high as 0.5.

 

Key words: Pseudokirchneriella subcapitata, spectrophotometry, ecotoxicological tests.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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