Journal of
Environmental Chemistry and Ecotoxicology

  • Abbreviation: J. Environ. Chem. Ecotoxicol.
  • Language: English
  • ISSN: 2141-226X
  • DOI: 10.5897/JECE
  • Start Year: 2009
  • Published Articles: 197

Short Communication

Measurement uncertainty for the determination of the inhibitory effect on Vibrio fischeri: A practical approach

Caterina Ledda*, Gea Oliveri Conti, Maria Fiore, Roberto Fallico, Salvatore Sciacca and Margherita Ferrante
Department G.F. Ingrassia- Hygiene and Public Health, University of Catania. Via Santa Sofia, 87- 95123 Catania-Italy.
Email: [email protected]

  •  Accepted: 24 January 2011
  •  Published: 31 May 2011

Abstract

 

The 11348-3:2007 ISO method allows the evaluation of the inhibitory effect of water samples on the light emission of Vibrio fischeri; it is essential to quantify the measurement uncertainty, but there is no available guideline. The aim is to come up with a practical approach to calculate the measurement uncertainty. We propose a test with different 3,5-dichlorophenol dilutions to evaluate if the luminometer is functioning properly and, after, we suggest to consider the uncertainty contributions sum (xi), to calculate the standard deviation of reproducibility (SR) and, finally, to express the expanded uncertainty with the formula U = 2SR + u(xi).

 

Key words: Vibrio fischeri, ISO 11348-3:2007, measurement uncertainty, microtox, uncertainty contributions.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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