Journal of
Entomology and Nematology

  • Abbreviation: J. Entomol. Nematol.
  • Language: English
  • ISSN: 2006-9855
  • DOI: 10.5897/JEN
  • Start Year: 2009
  • Published Articles: 135

Full Length Research Paper

Virulence of Beauveria bassiana against Sunn pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae) at different time periods of application

  Abdul Nasser Trissi1, Mustapha El Bouhssini2*, Mohammad Naif Al Salti1, Mohammad Abdulhai4, Margaret Skinner3 and Bruce L. Parker3
  1Aleppo University, Faculty of Agriculture, Aleppo, Syria. 2International Center for Agricultural Research in the Dry Areas, P. O. Box 5466, Aleppo, Syria. 3Entomology Research Laboratory, University of Vermont, Burlington, VT, USA. 05405-0105, USA. 4General Commission for Scientific Agricultural Research, Aleppo Center, Aleppo, Syria.  
Email: [email protected]

  •  Published: 31 August 2012



The virulence of Beauveria bassiana to overwintering Sunn pest adults was determined in the laboratory using micro-application techniques. Five microliter of 2 × 103, 2 × 104, 2 × 105, 2 × 106, 1 × 107, 2 × 107, 1 × 108 conidia/ml-1 were used in  November, January and March. Applications were made to the mesosternum of adults. Mortality was recorded every 2 days for 16 days. Isolates were efficacious to Sunn pest at the higher concentrations. The cumulative corrected mortality after 12 days varied from 2.3% (SPT22) at 2×103 conidia/ml-1, when treatment was conducted in November, to 100% (SPSR2) at 1×108 conidia/ml-1 in March. The LC50 for SPSR2 decreased from 1.1×107 to 3.7×103 conidia/ ml-1 when treatment was conducted in November and March, respectively. The 50% lethal time (LT50) varied from 17.07days for GHA (the commercial fungal isolate – in BotaniGard), to 9.31 days for SPSR2 in November. The most efficacious isolate was SPSR2.    


Key words: Sunn pest, Eurygaster integriceps, Beauveria bassiana, LC50, LT50.