Journal of
Entomology and Nematology

  • Abbreviation: J. Entomol. Nematol.
  • Language: English
  • ISSN: 2006-9855
  • DOI: 10.5897/JEN
  • Start Year: 2009
  • Published Articles: 130

Full Length Research Paper

Study of using the Bacterium Bacillus thuringiernsis israelensis in microbial control of Musca domestica vicina, Diptera Muscidae (Muscidae, Diptera)

Najlaa, Y. Abozinadah, Faten, F. Abuldahb, Nawal and S. Al- Haiqi
Department of Biology, Faculty of Science, King Abdul Aziz University, Saudi Arabia
Email: [email protected]

  •  Accepted: 28 March 2011
  •  Published: 30 April 2011

Abstract

 

The second instar larvae of Musca domestica were treated with Bacillus thuringiensis israelensis, (B.t.i.) at concentrations of 0.5, 1.0, 1.5 and 2.0% under laboratory conditions. Cumulative mortality percentage increased gradually with the increase in B.t.i concentrations and this is represented by straight regression lines indicating homogenity. The LC30 and LC50were 0.87 and 1.305%, respectively. The tested concentrations indicate significant prolongation in larval duration compared to control. There was an inverse relation between the concentration and pupation percentage and an increase in pupal duration. An insignificant decrease in percentage of adult emergence was observed. Sex ratio was not affected. 2.0% Concentration of B.t.i. caused the greatest reduction in female fecundity (964.5 eggs/female), and the hatchability of eggs also decreased. The pupae and adults appeared malformed when treated with B.t.i. as a 2nd larval instar.

 

Key words: Housefly, larvicide, bioassay, mortality, LC30, fecundity, fertility, malformation.