Journal of
Entomology and Nematology

  • Abbreviation: J. Entomol. Nematol.
  • Language: English
  • ISSN: 2006-9855
  • DOI: 10.5897/JEN
  • Start Year: 2009
  • Published Articles: 135

Full Length Research Paper

Isolation and structure determination of nematicidal iridoid sweroside from Alstonia scholaris

Nighat Sultana1*, Musarrat Akhter2, Zafar Saeed Saify3, Zakia Khatoon2, Mahmood-Ul-Hasan1, Muhammed Saleem Qazi4 and Yousaf Ali5
  1Pharmaceutical Research Center, PCSIR Laboratories Complex, Karachi-75280, Pakistan. 2Food and Marine Resources Research Centre, PCSIR Laboratories Complex, Karachi-75280, Pakistan. 3International Center for Chemical Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi 75270, Pakistan. 4 Departments of Biotechnology, University of Karachi, Karachi, Pakistan. 5Medicinal Botanic Centre, PCSIR Laboratories Complex, Jamrud Road, Peshawar, Pakistan.  
Email: [email protected]

  •  Accepted: 10 May 2013
  •  Published: 31 May 2013



The methanol extract of aerial parts of Alstonia scholaris (sapthaparna) was found to possess good nematicidal activity against the plant parasitic nematode, Meloidogyne incognita. The active nematicidal compound was isolated from the methanol extract by column chromatographic techniques and purified by TLC. Chemical structure was determined by chemical and spectroscopic methods to be that of a sweroside. This is the first report of its isolation from A. scholaris. Among other pure compounds: 4, 6 and 7, it showed maximum mortality of 71, 89 and 91%, respectively, after 24 h, while after 48 h, compounds 4, 6 and 7 showed 88, 74 and 76% mortality, respectively. The plant is of economic importance having nematicidal value. These results showed that the compounds isolated from A. scholaris have potent nematicidal activity and may be responsible for the antinematicidal activity of whole plant.


Key words: Meloidogyne incognita, Alstonia scholaris, iridoidApocynaceae, triterpenoids, nematicide.