Journal of
Engineering and Technology Research

  • Abbreviation: J. Eng. Technol. Res.
  • Language: English
  • ISSN: 2006-9790
  • DOI: 10.5897/JETR
  • Start Year: 2009
  • Published Articles: 198

Full Length Research Paper

Restriction analysis of rDNA regions to differentiate non-Saccharomyces wine species in mixed cultures

Capece A, Siesto G, Romaniello R and Romano P*
Dipartimento di Biologia, Difesa e Biotecnologie Agro-forestali, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy.
Email: [email protected]

  •  Accepted: 15 June 2009
  •  Published: 31 July 2009



The control of wine quality is related also to the knowledge of composition and dynamics of yeast-flora occurring throughout the vinification process. The restriction analysis of NTS and ITS regions was used to differentiate in mixed fermentation non-Saccharomyces species frequently encountered in the early fermentation phase, such as Hanseniaspora uvarum, Candida stellata and Metschnikowia pulcherrima. PCR-RFLP analysis of ITS region allowed to identify all the species inoculated in the grape must. The novelty of the protocol used is the application of PCR-RFLP analysis on yeast DNA isolated directly from grape must fermentation, without previous yeast colony isolation.


Key words: Non-Saccharomyces yeasts, NTS region, yeast mixed cultures.