Journal of
Engineering and Technology Research

  • Abbreviation: J. Eng. Technol. Res.
  • Language: English
  • ISSN: 2006-9790
  • DOI: 10.5897/JETR
  • Start Year: 2009
  • Published Articles: 184

Full Length Research Paper

Computational approach for comparative phylogenetic analysis of isolated chromium resistant strain Brevibacterium casei

Alok Prasad Das1* and Akalabya Bissoyi2
  1Centre of Biotechnology, Siksha ‘O’ Anusandhan University, Bhubaneswar, Odisha, India. 2National Institute of Technology, Rourkela, India.
Email: [email protected]

  • Article Number - 645766910731
  • Vol.3(3), pp. 82-87, March 2011
  •  Accepted: 10 February 2011
  •  Published: 31 March 2011

Abstract

 

The isolated (Cr [VI]) resistant bacterial strain was identified as Brevibacterium caseiby 16S rRNA sequencing (Genbank Accession Number: EU781952). The generated sequenced data was used for construction of Phylogenetic tree (MEGA 3.1and clustalw2) and predict the two dimensional alignment of the highly conserved regions. Computational comparative 16S rRNA sequence analysis of the isolated strain with different chromium resistant strains was carried out .The result shows that the compared sequence differs from the other especially in the region of 210 to 242, 440 to 480 nucleotides and had higher resemblance from 245 to 440, 500 to 724 in nucleotide base pair (in the Escherichia coli numbering system). From the overall study it can be predicted that the conserved region in the nucleotide base pair in all Cr (VI) resistant species may play an important role in chromium resistance.

 

Key words: Hexavalent chromium, 16S rRNA sequence, Brevibacterium casei, computational approach, phylogenetic analysis.

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