Journal of
General and Molecular Virology

  • Abbreviation: J. Gen. Mol. Virol.
  • Language: English
  • ISSN: 2141-6648
  • DOI: 10.5897/JGMV
  • Start Year: 2009
  • Published Articles: 36

Full Length Research Paper

First report of symptoms associated to a phytoplasma affecting Nopal (Opuntia ficusindica Mill) in the “Pyramids” Indian, State of Mexico

Ricardo Hernández-Pérez1, Juan Carlos Noa-Carrazana2*, Ricardo Gaspar3, Pedro Mata3 and Norma Flores-Estévez2
  1Center for Sustainable Agricultural Transformation. University of Cienfuegos, Cuatro Caminos, CP 59430, Cienfuegos, Cuba. 2Biotechnology and Applied Ecology Institute (INBIOTECA)., Veracruzana University. Culturas Veracruzanas No 101, Col E. Zapata CP 91090, Xalapa, Veracruz, México. 3Phytodiagnosis Laboratory, E. Zapata 10, San Luis Huexotla CP 56250 Texcoco, México.
Email: [email protected]

  •  Accepted: 17 September 2009
  •  Published: 31 December 2009

Abstract

 

An unknown symptomatology, including yellowing in old plantations, colorless of cladode epidermis, cladode deforming and curly, were observed on the Indian Fig (prickly pear cactus) (Opuntia ficus-indica Mill) in the “Pyramids” Indian area located in the northeastern State of Mexico. The samples collected were analyzed through a nested-PCR reaction. The symptoms found, were all attributed to the presence of a phytoplasma given the amplification of a 1200 pb fragment of the 16S rRNA gene using primers R16 F2/R2. The PCR products restriction analysis leading us to assume that the phytoplasma corresponds to subgroup 16Srll, previously reported for other crops in other world regions.

 

Key words: Indian fig, phytoplasma, Mexico state, 16S rRNA, PCR detection.