Full Length Research Paper
An unknown symptomatology, including yellowing in old plantations, colorless of cladode epidermis, cladode deforming and curly, were observed on the Indian Fig (prickly pear cactus) (Opuntia ficus-indica Mill) in the “Pyramids” Indian area located in the northeastern State of Mexico. The samples collected were analyzed through a nested-PCR reaction. The symptoms found, were all attributed to the presence of a phytoplasma given the amplification of a 1200 pb fragment of the 16S rRNA gene using primers R16 F2/R2. The PCR products restriction analysis leading us to assume that the phytoplasma corresponds to subgroup 16Srll, previously reported for other crops in other world regions.
Key words: Indian fig, phytoplasma, Mexico state, 16S rRNA, PCR detection.
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