Journal of
Infectious Diseases and Immunity

  • Abbreviation: J. Infect. Dis. Immun.
  • Language: English
  • ISSN: 2141-2375
  • DOI: 10.5897/JIDI
  • Start Year: 2009
  • Published Articles: 93

Full Length Research Paper

An immunoassay using cord factor antigen of Mycobacterium tuberculosis for the confirmatory diagnosis of intracranial tuberculoma

Mathai A., Neelima R., Harikrishnan V. S. and Radhakrishnan V. V.*
Division of Laboratory Animal Science Biomedical Technology, Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum- 695 011, Kerala State, India.
Email: [email protected]

  •  Accepted: 21 March 2011
  •  Published: 30 April 2011


Demonstration of Mycobacterium tuberculosis bacillus in an intracranial tuberculoma by  bacteriological culture and staining methods are difficult. Hence the objective of this study is to describe an immunoassay to detect the presence of cord factor antigen of M.tuberculosis in intracranial tuberculoma. Cord factor antigen of M. tuberculosis in the surgical specimens of intracranial tuberculoma was isolated by a chloroform – methanol precipitation method and its concentration was quantitated by a direct enzyme-linked-immunosorbent assay (ELISA). Cord factor antigen of M. tuberculosis was present in the intracranial tuberculous lesions and absent in intracranial fungal granulomas. Demonstration of cord factor antigen of M. tuberculosis as described in this study is unique and may be able to distinguish tuberculous from fungal etiology in patients with intra- cranial granulomatous lesions. Thus it has a potential application in the laboratory diagnosis of intracranial tuberculoma.


Key words: Intracranial tuberculoma, Mycobacterium tuberculosis, cord factor antigen, specificity, enzyme linked immunosorbent assay.