Journal of
Medical Genetics and Genomics

  • Abbreviation: J. Med. Genet. Genomics
  • Language: English
  • ISSN: 2141-2278
  • DOI: 10.5897/JMGG
  • Start Year: 2009
  • Published Articles: 48

Full Length Research Paper

Screening of g.IVS5+1G to a mutation of TG gene and thyroid hormone level among Iraqi thyroid disorders

AL-Ramahi I. J.1, AL-Faisal A. H. M.2*, Abudl-Reda I.2, Aouda N.3, AL-Atar R.4and Barusrux S.5
1Ministry of Industry and Minerals-General commission for Research and Industrial Development, AL-Razi Center for Research and Medical Diagnostic Kits Production, Iraq. 2Genetic Engineering and Biotechnology Institute(GEBI), Baghdad, Iraq. 3Endocrinologist, AL Yarmouk Teaching Hospital, Nuclear Medicine Branch, Baghdad, Iraq. 4Endocrinologist, Hospital of Radiotherapy and Nuclear Medicine, Baghdad, Iraq. 5Center for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002,Thailand.
Email: [email protected]

  •  Published: 31 January 2011


TG gene mutations have been identified in some people who have a goiter but normal or boarder line thyroglobulin levels. These mutations are either caused by gene deletion or single nucleotide mutation as a result. This study aim to screen the frequency of g.IVS5+1G to A mutation of TG gene and thyroid hormone levels among Iraqi thyroid disorders. g.IVS5+1G to A mutation of TG gene and thyroid hormones levels were detected in Iraqi thyroid disorders including 16 metastatic follicular thyroid carcinoma, 31 toxic goiter, 32 non toxic goiter and 19 hypothyroidism in addition to 25 normal control. The g.IVS5+1G to A mutation of TG gene screened by LAN-PCR. The heterozygous point mutation g.IVS5+1G  to A was detected at position +1 of the splice donor site of intron 5 in 57% of cancer patients, 55% of toxic goiter and in 44% non toxic goiter patients but not in patients with hypothyroidism. The results also showed no correlation between hormones levels and detected mutation. We conclude that the g IVS5+1G>A mutation which caused fusion  of  exons 4 and 6 was detected in high frequency in Iraqi patients with thyroid disorders and no correlation was found between mutation and the levels of the thyroid hormones.


Key words: TG gene, thyroid cancer, mutation, goiter.