Journal of
Medical Laboratory and Diagnosis

  • Abbreviation: J. Med. Lab. Diagn.
  • Language: English
  • ISSN: 2141-2618
  • DOI: 10.5897/JMLD
  • Start Year: 2010
  • Published Articles: 54

Full Length Research Paper

Total and CD4+ T- lymphocyte count correlation in newly diagnosed HIV patients in resource-limited setting

Fasakin KA
  • Fasakin KA
  • Department of Haematology, Federal Medical Centre, P.M.B 201, Ido Ekiti, Ekiti State, Nigeria.
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Omisakin CT
  • Omisakin CT
  • Department of Haematology, Federal Medical Centre, P.M.B 201, Ido Ekiti, Ekiti State, Nigeria.
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Esan AJ
  • Esan AJ
  • Department of Haematology, Federal Medical Centre, P.M.B 201, Ido Ekiti, Ekiti State, Nigeria.
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Adebara IO
  • Adebara IO
  • Adebara Idowu Oluseyi, Department of Obstetrics and Gynaecology, Federal Medical Centre, P.M.B 201, Ido Ekiti, Ekiti State, Nigeria.
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Owoseni IS
  • Owoseni IS
  • Alpha Medical Laboratory, Off Ondo Road, Akure, Ondo State, Nigeria.
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Omoniyi DP
  • Omoniyi DP
  • Department of Haematology, Federal Medical Centre, P.M.B 201, Ido Ekiti, Ekiti State, Nigeria.
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Ajayi OD
  • Ajayi OD
  • Department of Haematology, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife.
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Ogundare RG
  • Ogundare RG
  • Department of Medical Microbiology, Federal Medical Centre, P.M.B 201, Ido Ekiti, Ekiti State, Nigeria.
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Moronkeji MB
  • Moronkeji MB
  • Department of Chemical Pathology, Ladoke Akintola University of Technology Teaching Hospital, Osogbo, Osun State, Nigeria.
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  •  Accepted: 21 February 2014
  •  Published: 28 February 2014

Abstract

Few clinical settings in resource-limited countries perform CD4+ T-lymphocyte counts required as a baseline test for antiretroviral therapy. We investigated CD4 count in newly diagnosed HIV-infected patients attending our treatment centre and evaluated suitability of total lymphocyte count (TLC) as a surrogate marker for CD4+T-lymphocyte count required as a yardstick for initiating antiretroviral therapy. Usefulness of TLC as a surrogate marker for  CD4+T-lymphocyte counts  <200, ≤350 and <500cells/µL for HIV-positive patients in our facility was evaluated by 180 pairs of TLC and CD4  counts from 180 newly diagnosed HIV-infected patients and results were compared by linear regression and Spearman’s correlation analytical tools. Approximately 72.8% of our patients were diagnosed late as revealed by CD4 count ≤350cells/µL. An overall good correlation was noted between TLC and CD4+T-cell counts (r=0.65, slope=0.69), mean total lymphocyte count of 1.04 ± 0.81, 1.39 ± 1.06 and 1.57 ± 1.13 x 10⁹/L correspond to CD4 lymphocyte counts of <200, ≤350 and < 500cells/µL respectively. When considering initiating HAART for HIV-infected Nigerian clients, TLC can be considered as an inexpensive and easily accessible surrogate marker for predicting CD4+T-lymphocyte at two clinically important CD4 thresholds of CD4 count of ≤350 cells/µL and <500cells/µL.

Key words: CD4, total lymphocyte count, highly active antiretroviral therapy, enzyme immunoassay, HIV, flowcytometry.