Journal of
Medical Laboratory and Diagnosis

  • Abbreviation: J. Med. Lab. Diagn.
  • Language: English
  • ISSN: 2141-2618
  • DOI: 10.5897/JMLD
  • Start Year: 2010
  • Published Articles: 60

Full Length Research Paper

Towards the development of rapid biofilm antibiotic sensitivity testing (BAST)

AL Quthami Khalid
  • AL Quthami Khalid
  • Regional Laboratory,MOH, Makkak, Saudi Arabia.
  • Google Scholar
Bassam O. AlJohny
  • Bassam O. AlJohny
  • Biological Science Department, Faculty of Science, King Abdulaziz University, P, O, Box 80203, Jeddah 21589, Kingdom of Saudi Arabia
  • Google Scholar
Milton Wainwright
  • Milton Wainwright
  • Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, United Kingdom.
  • Google Scholar

  •  Accepted: 09 September 2013
  •  Published: 31 October 2013


This study presents a modification of the antibiotic susceptibility testing (AST), which is a rapid means of determining the response of planktonic bacteria to different antimicrobial agents, for application to biofilms. Colony biofilm was first developed on a cellulose filter/membrane disc, over which an antibiotic disc was imposed. Zone of inhibition was measured after incubation on nutrient agar. Biofilms were not as susceptible to the test antibiotics as compared to the planktonic cultures. The results point to the possibility of this method as a rapid means for antibiotics for treating biofilm infections. Limitations and potential application for biofilm AST are discussed.


Key words:  Biofilm, antibiotic susceptibility testing