September 2010
Chemical composition, antimicrobial and antioxidant activity of essential oil of endemic Ferula lycia Boiss
The essential oil of Ferula lycia Boiss. was analyzed by GC-MS and assayed for its antimicrobial activity. Thirty six components representing 96.76% of the total oil were characterized. The most prominent component of oil was α-pinene (59.89%). β-Pinene (19.01%), limonene (3.21%) and bornyl acetate (2.10%) were the other main constituents of the oil. To evaluate the antimicrobial activity of...
September 2010
Quantiï¬cation of xanthones in a Mongolian health tea using high-performance liquid chromatography
Gentianella acuta, known as “guixincao”, has been used as a health tea with the properties of clearing heat and toxic materials, removing pathogenic heat from blood, and increasing secretion of urine. In this study, high-performance liquid chromatography (HPLC) equipped with ultraviolet (UV) detection was used for quantiï¬cation of two major bioactive xanthones in the “guixincao” tea...
September 2010
Promoting scopolamine accumulation in transgenic plants of Atropa belladonna generated from hairy roots with over expression of pmt and h6h gene
Atropa belladonna is the most important commercial source for obtaining pharmaceutical tropane alkaloids such as scopolamine and hyoscyamine. In the present study, two rate-limiting enzyme genes including putrescine N-methyltransferase and hyoscyamine 6β-hydroxylase were introduced into A. belladonna, and integration of the pmt and h6h genes into the genomic DNA of transgenic...
September 2010
Mutational approach for enhancement of artemisinin in Artemisia annua
The potential mutagenic effect of sodium azide (NaN3) was studied on seeds and callus of Artemisia annua for enhancement of artimisinin. The treated and untreated seeds were germinated with half MS liquid medium and the leaves (two-weeks-old) were employed to raise callus on full strength of medium containing α-naphthalene acetic acid (NAA) and kinetin (each 0.5 mg/L). The mutant calluses were...
September 2010
Phenolic contents, antioxidant and antimicrobial activity of Papaver rhoeas L. extracts from Southeast Serbia
Total phenolic and flavonoid contents of different extracts of Papaver rhoeas L. were examined. High contents of total phenolic compounds (9.73 -19.91 mg GAE/g of fresh petals) and total flavonoids (7.904 - 11.45 mg QE/g of fresh petals) were determined. Red pigment present in the flowers of P. rhoeas L. originates from anthocyanins, which may act as natural antioxidants. Anthocyanins content in the...
September 2010
Effects of foliar application of micronutrients (Fe and Zn) on flower yield and essential oil of chamomile (Matricaria chamomilla L.)
In order to evaluate the effects of foliar application of micronutrients (iron and zinc) on yield and essential oil of chamomile, two field experiments were carried out in 2008 and 2009 at the Research Station of Faculty of Agriculture, University of Tabriz, Iran. Both experiments were arranged as factorial on the basis of randomized complete block design (RCBD) with four replications. Treatments were foliar...
September 2010
In-vitro studies on lectin derivatives of Aloe excelsa (Berger)
Current traditional remedies for cure in various ailments including skin treatments have been on the increase. Aloe excelsa has been noted to be used extensively by indigenous people for sun burns, burns, sores as well as a systemic remedy. The agglutination activity by A. excelsa has been credited to the presence of lectins or lectin like derivatives. Aloctin A from A. excelsa was tested...
September 2010
Effects of ultrasonic-assistant extraction parameters on total flavones yield of Selaginella doederleinii and its antioxidant activity
This paper, applied the central composite design combined with response surface methodology to optimize the parameter of ultrasonic-assisted extraction the total flavonoids from Selaginella doederleinii Hieron (SD). The results indicate that themaximum extract of total flavonoids was 4.414 mg/g by using 70% ethanol as solvent, extract 50 min at 65°C....
September 2010
Ethnobotanical and pharmaceutical evaluation of Capparis spinosa L, validity of local folk and Unani system of medicine
This paper describe ethnobotanical and pharmaceutical importance of Capparis spinosa L, (Capparidaceae) used by many local people since century under the traditional, Ayurvedeic, Chinese and Unani system of medicine. The present endeavour was, therefore, conducted during summer 2010 with the aim to document and prepare detail ethnobotanical inventory and pharmaceutical importance of C. spinosa and...
September 2010
Screening for antidiabetic activity and phytochemical constituents of common bean (Phaseolus vulgaris L.) seeds
Seeds of Phaseolus vulgaris were given individually at different doses to different batches of rats (both normal and hyperglicemic rats) after an overnight fast. Seeds contain the bioactive components – alkaloids, flavonoids, fiber, proteins, tannins, terpenoids, saponins, quercetin, anthocyanin and catechin. The blood glucose levels were measured at 0, 1, 2, 3, 4, 5 and 6 h after the treatment. Most...
September 2010
Evaluation of antioxidant, immunomodulating, cytotoxic and antimicrobial properties of different strains of Basidiomycetes from Northeastern Mexico
A great variety of mushrooms (Basidiomycetes) grow in the Mexican territory, but most of their properties, including pharmacological ones, have been scarcely investigated. Samples of culture broth of ten different strains of Mexican Basidiomycetes cultured in four growing times were taken and lyophilized; then, their antioxidant, immunomodulating, cytotoxic and antimicrobial properties were studied, the antioxidant...
September 2010
Comparative in vivo evaluation of the trypanocidal activities of aqueous leaf, stem-bark and root extracts of Khaya senegalensis on Trypanosoma evansi
The effects of three different parts of Khaya senegalensis, commonly used in the traditional treatment of diseases in Northern Nigeria was examined in Trypanosoma evansi infected rats. At a dose of 120 mg/ml body weight for 3 consecutive days, the aqueous stem bark extract completely suppressed parasite establishment. The dose cured the experimentally infected rats in 9 days. The aqueous leaf extract showed a weak...
September 2010
Optimization of protoplast isolation protocols from callus of Eurycoma longifolia
Protoplast technology offers a unique single cell system that facilitates several aspects of modern biotechnology. In this study, an efficient protocol to isolate the protoplast from callus culture of a valuable medicinal plant in Southeast Asia,Eurycoma longifolia was developed. A range of parameters which influence the isolation of E. longifolia protoplasts were investigated by using...
September 2010
Forest resource utilization assessment for economic development of rural community in northern parts of Pakistan
A study on the ethnobotanical investigation of some multipurpose fruit plants was conducted in three important sites of District Swat of Pakistan during summer, 2008. The aim of the study was to identify the multipurpose fruit plants growing in the area and to collect information on their uses by the local people. Ethnobotanical information was collected through the show-and-tell/semi-structured method and personal...
September 2010
Use of traditional medicinal plants by indigenous people in Mekele town, capital city of Tigray regional state of Ethiopia
An ethno-botanical study of traditional medicinal plants used by indigenous people in Mekele city, northern Ethiopia was carried out from July - September 2009. Thirteen informants between the ages of 18 and 56 were randomly selected for the study. Ethno-botanical data were collected using semi-structured interview and field observations. The routes of administration were oral and dermal with equal proportions. The...
September 2010
Antibacterial activity of elder (Sambucus nigra L.) flower or berry against hospital pathogens
An evidence-based scientific scrutiny of Irish traditional medicines for their antimicrobial potency is urgently required for combating antibiotic resistant common nosocomial pathogens. We now report our seminal findings on the major constituents including terpenes identified in native, historically significant herbal medicinal plant Elder (Sambucus nigra L.) flower and elder berry in...
September 2010
Composition and antifungal activity against Candida albicans, Candida parapsilosis, Candida krusei and Cryptococcus neoformans of essential oils from leaves of Piper and Peperomia species
This study was addressed to investigate the composition and antifungal activity of essential oils from leaves of Piperaceae species (Piper aduncum, P. amalago, P.cernuum, P. diospyrifolium, P. crassinervium, P. gaudichaudianum, P. solmsianum,P. regnellii, P. tuberculatum, P. umbelata and Peperomia obtusifolia)...
September 2010
Traditional uses of medicinal plants in Darra Adam Khel NWFP Pakistan
The present study documents the traditional knowledge of medicinal plant species used in Darra Adam Khel NWFP Pakistan. We have documented the use of 34 species belonging to 25 families. The dominant families are Lamiaceae with 3 species, followed by Monaceae with 3 species, Mimosaceae, Apocynaceae, Asteraceae and Liliaceae with 2 species each. These medicinal plants are used to cure about 30 - 35 types of ailment. The...
September 2010
Detection of somaclonal variations using RAPD fingerprinting in Silybum marianum (L.)
Random amplification of polymorphic DNA (RAPD) markers was used to detect somaclonal genetic variations between in vitro and in vivo grown tissues of Silybum “S. marianum”. Ten primers (OPC1-OPC10) of RAPD OPC were used. All the primers gave reproducible banding pattern except OPC3. Monomorphic bands were observed in case of all primers, whereas only OPC10...
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