Journal of
Petroleum Technology and Alternative Fuels

  • Abbreviation: J. Pet. Technol. Altern. Fuels
  • Language: English
  • ISSN: 2360-8560
  • DOI: 10.5897/JPTAF
  • Start Year: 2010
  • Published Articles: 69

Full Length Research Paper

Biodiesel production from marine microalgae Chlorella marina and Nannochloropsis salina

A. Muthukumar*, S. Elayaraja, T. T. Ajithkumar, S. Kumaresan and T. Balasubramanian
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai -608502, Tamil Nadu, India
Email: [email protected]

  •  Accepted: 30 July 2012
  •  Published: 31 October 2012



The aim of the study was to obtain high quality biodiesel from microalgae Chlorella marina and Nannochloropsis salina through transesterification. Growth studies revealed that maximum cell growth rate was obtained at 15th day of the culture. The flocculation activity result showed that pH 11 was optimum for cell flocculation at 37°C. In the present study, 60.26% of biodiesel yielded from 0.752 g L-1 contains 30% oil content from N. salina, whereas 50% yielded from 0.527 g L-1 contains 20% oil content. The crude lipid content found in C. marina and N. salina was found to be 20.33 ± 1.82% and 32.13 ± 1.40% of dry biomass. The density and viscosity of the biodiesel obtained from the crude lipid of N. salina and C. marina were 0.992 and 0.971 (kg L-1), viscosity 3.2 and 4.8 (Pa.sat 40°C), respectively. The method implemented in this study could be novel approach and great potential in the industrial production of liquid fuel from microalgae.


Key words: Microalgae, Nannochloropsis salina, Chlorella marina, lipids, biodiesel, flocculation