Research in Pharmaceutical Biotechnology

  • Abbreviation: Res. Pharm. Biotech.
  • Language: English
  • ISSN: 2141-2324
  • DOI: 10.5897/RPB
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

Antinociceptive potential of the methanolic extract of Microdermis puberula (Hook. F. Ex. Planch)

Clement Jackson
  • Clement Jackson
  • Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Nigeria.
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Herbert Mbagwu
  • Herbert Mbagwu
  • Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Nigeria.
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Chidinma Okany
  • Chidinma Okany
  • Department of Pharmacology, College of Medicine University of Lagos, Nigeria.
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Emmanuel Bassey
  • Emmanuel Bassey
  • Department of Pharmacology, College of Medicine University of Lagos, Nigeria.
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Iniunwana Udonkang
  • Iniunwana Udonkang
  • Department of pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Uyo, Nigeria.
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  • Article Number - F485BB5469
  • Vol.3(5), pp. 53-56, May 2011
  •  Accepted: 22 March 2011
  •  Published: 30 May 2011

Abstract

 

This study investigated the antinociceptive effects of the methanolic extract of the stem wood ofMicrodermis puberula (Hook. F. Ex. Planch) in Swiss albino mice of either sex with weights ranging from 17  to 25 g. Acetylsalicylic acid and morphine were used as standards for the antinociceptive tests while distilled water was used as control. Acetic acid - induced mouse writhing reflex test and hot plate test were used to determine peripheral and central pain inhibition respectively, using five groups of five animals for each model. The extract was administered in three dosages: 600, 1200 and 2400 mg/kg orally. Acetylsalicylic acid was administered at 100 mg/kg p.o., morphine at 10 mg/kg intraperitoneally and distilled water at 1 ml/kg p.o. Results showed that the extract of. Microdermis puberula, at 1200 and 2400 mg/kg significantly inhibited peripheral pain, compared with acetylsalicylic acid (p<0.05). The extract of Microdermis puberula, at a dose of 2400 mg/kg inhibited pain by increasing the pain threshold in the hot plate test 58.83%. These, therefore, suggest that the methanolic extract ofMicrodermis puberula possesses mild antinociceptive property. The phytochemical analyses of the extract showed the presence of flavonoids, saponins and cardiac glycosides. Results from the LD50 determination showed that a dose of 1412.5 mg/kg i.p. produced 50% death in mice.

 

Key words: Antinociceptive, Microdemis puberula, phytochemical analysis, LD50, acetylsalicylic acid.