African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Nutrient effects on production of cellulolytic enzymes by Aspergillus niger

Narasimha G1, Sridevi A1, Buddolla Viswanath2, Subhosh Chandra M2, Rajasekhar Reddy B2*
2Department of Microbiology, Sri Krishnadevaraya University, Anantapur-515 003. Andhra Pradesh, India. Present Address: 1 National P.G. College, Nandyal, Andhra Pradesh, India.
Email: [email protected]

  •  Accepted: 25 August 2005
  •  Published: 01 March 2006

Abstract

The production of cellulase (filter paper activity, endoglucanase and β-glucosidase) by Aspergillus niger on three media in liquid shake culture was compared. The culture filtrate of this organism exhibited relatively highest activity of all three enzymes and extracellular protein content at 7-day interval during the course of its growth on Czapek-Dox medium supplemented with 1.0% (w/v) cellulose. Urea as a nitrogen source and pH 5.0 were found to be optimal for growth and cellulase production by A.niger. Among various soluble organic carbon sources and lignocelluloses tested in this study, carboxymethylcellulose and sawdust at 1% supported maximum production of all three enzymes by A. niger.

 

Key words: Aspergillus niger, cellulase activity, nutrients, β-glucosidase.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 5 No. 5
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES