African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12484

Full Length Research Paper

Analysis of AVR4 promoter by sequential response-element deletion

Olukosi, YA1 and Iwalokun, BA2*
  1Genetics Division, Nigerian Institute of Medical Research (NIMR), 6, Edmond Crescent, Yaba – Lagos, PMB 2013, Lagos - Nigeria. 2Biochemistry Dept, Lagos State University, PMB 1087, Apapa – Lagos, Nigeria.
Email: [email protected]

  •  Accepted: 24 June 2003
  •  Published: 31 July 2003

Abstract

 

Several reports have associated the variability in physico-chemical properties of avidin protein to dynamism inherent in the consensus regulatory networks within the promoter region of avidin genes. An Avr4 promoter region ligated to chloramphenicol acetyltransferase plasmid vector (pBLCAT2) to produce recombinant plasmid Avr4pBLCAT2 was sequentially deleted to produce five distinct mutants: Avr4pBLCAT2907-176, Avr4pBLCAT2809-176, Avr4pBLCAT2789-176,Avr4pBLCAT2429-176 and Avr4pBLCAT2302-176. The transformants elicited different chloramphenicol acetyl transferase (CAT) activities.

 

Key words: Avidin, AVR4 promoter, chloramphenicol acetyl transferase.  

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This article is published under the terms of the Creative Commons Attribution License 4.0

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