African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Monechma ciliatum methanolic extract regulates low density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase genes expression in HepG2 cells

Abdalbasit Adam Mariod1,3, Ghanya AL-Naqeb1,4 and Maznah Ismail1,2*
1Laboratory of Molecular Biomedicine, Institute of Bioscience, University PutraMalaysia, 43400 Serdang, Selangor, Malaysia. 2Department of Nutrition and Dietetic, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 3Department of Food Science and Technology, Sudan University of Science and Technology, P. O Box 71, Khartoum North, Sudan. 4Department of Food Science and Technology, Faculty of Agriculture, University of Sana’a, Sana’a, Yemen.
Email: [email protected], [email protected].

  •  Accepted: 21 May 2010
  •  Published: 06 September 2010

Abstract

Monechma ciliatum methanolic extract (MCME) obtained from Monechma ciliatumseedcake showed high total phenolic compounds with high antioxidant activity. The regulatory effects of MCME at 10, 20 and 50 µg/ml on low density lipoprotein receptor (LDLR) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) were investigated in human HepG2 cell line using quantitative real-time polymerase chain reaction. LDLR mRNA level was increased significantly by 1.4, 2.6 and 4.3 fold in MCME treated cells at 10, 20 and 50, respectively, compared to untreated cells. Whereas, HMGCR mRNA level was decreased significantly by 38, 63 and 80% in MCME treated cells at 10, 20 and 50, respectively, compared to untreated cells. The effect of MCME was concentration dependent, and different doses showed significant differences in regulation of both LDLR and HMGCR genes. The present study showed that MCME effectively regulated the expression of LDLR and HMGCR genes influencing the cholesterol  metabolism in HepG2 cells.

 

Key words: Antioxidant activity, ß‑carotene-linoleic acid assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH), gene expression, low density lipoprotein receptor, 3-hydroxy-3-methylglutaryl-coenzyme A reductase, Monechma ciliatum.

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