African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Pyramiding of blast and bacterial leaf blight resistance genes into rice cultivar RD6 using marker assisted selection

W. Pinta1, T. Toojinda2, P. Thummabenjapone1 and J. Sanitchon3,4*
1Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University Khon Kaen, 40002, Thailand. 2Rice Gene Discovery Unit, Kasetsart University, Kanphaeng Saen, Nakhon Pathom, 73140, Thailand. 3Center of Excellence on Agricultural Biotechnology: (AG-BIO PERDO-CHE), Bangkok 10900, Thailand and Agricultural Biotechnology Research Center for Sustainable Economy, Khon Kaen University, Khon Kaen 40002. 4Plant Breeding Research Center for Sustainable Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand.
Email: [email protected]

  •  Accepted: 14 June 2013
  •  Published: 31 July 2013

Abstract

Blast caused by the fungus Magnaporthe oryzae (Hebert) Barr. and bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) are two major diseases of rice (Oryza sativa). The use of varietal resistance is the most appropriate strategy for controlling the diseases, and molecular assisted selection can potentially accelerate breeding programs. The objective of this study was to pyramid genes conferring resistance to blast and bacterial leaf blight diseases to rice cultivar RD6, using molecular assisted selection. Near-isogenic lines (NIL) derived from two blast resistant crosses (RD6 × P0489 and RD6 × Jao Hom Nin) were pyramided with IR62266 (xa5), to transfer bacterial leaf blight resistance to RD6 introgression lines. Five flanking sets of simple sequence repeat (SSR) markers (RM319/RM212, RM48/RM207, RM224/RM144, RM313/RM277 and RM122/RM159: 4 for blast and 1 for BLB resistance) were used for screening of introgression lines carrying 5quantitative trait loci (QTLs) from the BC1F2 generation through to BC2F2:3generation, and 12 pyramiding lines were identified. Gene validation for blast and bacterial leaf blight diseases was accomplished using artificial inoculation under greenhouse conditions. BC2F2:3 2-8-2-24 and BC2F2:3 2-8-2-25 showed greater levels of blast broad spectrum resistance (BSR) whereas BC2F2:3 2-8-2-36 expressed the highest of bacterial leaf blight resistance with a high blast BSR.

Key words: Gene pyramiding, introgression lines, molecular marker, Near-isogenic lines, SSR.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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