African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Genetic variation patterns of Medicago ruthenica populations from Northern China

RONG Yu-ping
  • RONG Yu-ping
  • Department of Grassland Science, Animal Science and Technology College, China Agricultural University, Beijing 100193, China.
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CAO Zhe
  • CAO Zhe
  • Department of Grassland Science, Animal Science and Technology College, China Agricultural University, Beijing 100193, China.
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ZHU Ling-ling
  • ZHU Ling-ling
  • Department of Grassland Science, Animal Science and Technology College, China Agricultural University, Beijing 100193, China.
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ZHANG Yong-juan
  • ZHANG Yong-juan
  • Department of Grassland Science, Animal Science and Technology College, China Agricultural University, Beijing 100193, China.
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BAI Ke-yu
  • BAI Ke-yu
  • Institute of Agricultural Resources and Regional Planning, China Academy of Agricultural Sciences, Beijing, 100081, China.
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  •  Accepted: 28 February 2012
  •  Published: 15 March 2012

Abstract

 

As a promising legume for forage in arid and semi-arid areas, Medicago ruthenica (L.) Trautv. is widely distributed in northern China. For this study, twenty four accessions from northern China were analyzed by allozyme and microsatellites markers to determine its genetic variation patterns, two assays were found different in the amounts of polymorphism. Higher amounts of polymorphism were detected by microsatellites. For allozymes and microsatellite markers, mean expected heterozygosity (He) of 24 populations ranged from 0.160 to 0.485 and 0.274 to 0.5316 respectively. Mean observed heterozygosity per locus (Ho) of 24 populations was 0.422 for allozymes and 0.4149 for simple sequence repeats (SSR) markers. For allozymes, average interpopulation genetic diversity (Hs) was 0.2733 and average intrapopulation genetic diversity (DST) was 0.0473, mean coefficient of gene differentiation for loci (GST) at the population level was estimated 0.1813. According to GST, gene flow at population level was 1.4427 for allozymes and 1.2040 for SSR markers, which indicated there was definite gene flow between populations. Low differentiations among populations at allozymes (FST = 0.1719) and SSR markers (FST = 0.1816) means genetic variations is mainly lied in populations. The results showed there was high genetic diversity in M. ruthenica populations.

 

Key words: Medicago ruthenica, genetic diversity, allozyme, micro-satellite, North China.

Abbreviation

DST, Genetic diversity; Hs, interpopulation genetic diversity; He,expected heterozygosity; GST, coefficient of gene differentiation for loci; SSR, simple sequence repeats.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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