African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

In vitro response from cotyledon and hypocotyls explants in tomato by inducing 6-benzylaminopurine

Abd-almajid Nasher Mohamed1*, Mohd Razi Ismail2 and Mohamed Hasan Rahman3
1Institute of Tropical Agriculture, University of Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 2Faculty of Agriculture, University of Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 3Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.
Email: [email protected]

  •  Accepted: 04 June 2010
  •  Published: 31 July 2010

Abstract

Differential response of tomato was evaluated by hypocotyls and cotyledon explants on 6-benzylaminopurine (BAP) containing MS media. Among the four levels (0, 1, 2 and 4 mg/l) of BAP employed in Murashige and Skoog (MS) media, 2.0 mg/l BAP was found superior in growth traits (number of shoots/explants and shoot length) and cultivars. No significant difference was noticed between cotyledon and hypocotyls explants on media having 2.0 mg/l BAP. In the same treatment, shoot length was significantly different between cotyledon and hypocotyls derived explants. No adventitious shoots was noted in the control and higher concentration of BAP containing media but the explants turned into callus on media having 4.0 mg/l BAP.

 

Key words: Cotyledon, hypocotyls, in vitro, tomato, 6-benzylaminopurine.

Abbreviation

BAP, 6-Benzylaminopurine; IAA, indole-3-acetic acid; Kin, kinetin;MS, Murashige and Skoog.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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