African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12482

Full Length Research Paper

Seperation, identification and analysis of pigment (melanin) production in Streptomyces

Dastager S.G.1, 2, Wen-Jun Li 1, Dayanand A.2, Shu-Kun Tang1, Xin-Peng Tian1 Xiao-Yang Zhi1, Li-Hua Xu1 and Cheng-Lin Jiang1
1Laboratory for conservation and utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan-650 091, P.R.China.. 2Department of Studies and Research in Microbiology. Gulbarga University, Gulbarga-585 106, Karnataka, India.
Email: [email protected]

  •  Accepted: 11 January 2006
  •  Published: 02 June 2006

Abstract

Nine strains among 180 Streptomyces isolates produce a diffusible dark brown pigment on both peptone-yeast extract agar and synthetic tyrosine-agar. They also show the positive reaction to L-tyrosine or L-dopa substrates. The pigment has been referred to be as merely as dark brown water-soluble pigment, as melanoid or melanin. The different carbon and nitrogen sources which influence the pigment production in the Streptomyces isolates were also investigated, and the carotenoid content in the pigment was analyzed. The melanin formation in the Streptomycesspecies is the key feature for the classification of the Stretomyces group.

 

Key words: Pigment, melanin, Streptomyeces, taxonomy.