Optimization of solid-state fermentation conditions for the production of cellulase and its hydrolytic potentials by Trichoderma virride Sn-9106

Guo Longwei; Chen Hongman*; Wang Huihui; Kan Guoshi; Ren Daming

doi:10.5897/AJMR2013.5764

African Journal of
Microbiology Research

Abbreviation: Afr. J. Microbiol. Res.
Language: English
ISSN: 1996-0808
DOI: 10.5897/AJMR
Start Year: 2007
Published Articles: 5233

Full Length Research Paper

Optimization of solid-state fermentation conditions for the production of cellulase and its hydrolytic potentials by Trichoderma virride Sn-9106

Guo Longwei

Guo Longwei
School of Life Science, Shenyang Agriculture University, Shenyang 110866, China.
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Chen Hongman*

Chen Hongman*
School of Life Science, Shenyang Agriculture University, Shenyang 110866, China.
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Wang Huihui

Wang Huihui
School of Life Science, Shenyang Agriculture University, Shenyang 110866, China.
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Kan Guoshi

Kan Guoshi
School of Life Science, Shenyang Agriculture University, Shenyang 110866, China.
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Ren Daming

Ren Daming
School of Life Science, Shenyang Agriculture University, Shenyang 110866, China.
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Article Number - 1BA442145567
Vol.8(26), pp. 2547-2554 , June 2014
https://doi.org/10.5897/AJMR2013.5764

Received: 26 March 2013
Accepted: 02 June 2014
Published: 25 June 2014

Copyright © 2024 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0.

Abstract

Trichoderma viride Sn-9106 with high cellulase activity was used to produce enzyme on residues of Chinese herbs as substrate in solid state fermentation. Residues of Chinese herbs and peptone were found to be the best combination of carbon and nitrogen source for the production of cellulase. The nutrient composition of medium was optimized using response surface methodology. A fractional factorial design (3³) was applied to elucidate the nutrient medium components that significantly affect cellulase production. The concentration of peptone and wheat bran in the medium was a significant factor. The composition of nutrient fermentation medium optimized with response surface methodology was in g/L: wheat bran, 19.8, peptone, 2.06 and KH₂PO_4, 2.9. Compared to the original medium, the cellulase activity increased from 3.8 to 7.5 IU/mL.

Key words: cellulase, Trichoderma viride Sn-9106, response surface methodology (RSM), solid state fermentation (SSF), residues of Chinese herbs (RCH).

Abbreviation

RSM, response surface methodology; SSF, solid-state fermentation; RCH, residues of Chinese herbs.