Abstract

Emissions of biological contaminants (microbes) from intensive pig farming may cause environmental problems due to lack of proper waste management. This work was conducted to assess bacteriological pollution of soil in pig farm and to detect the presence of antibiotic resistance gene of the prevailing bacteria. Soil samples were collected from March to August 2013. The method included bacterial enumeration (10 ^-1 to 10 ^-8) in Nutrient, Xylose Lysine Deoxycholate (XLD), Eosin Methylene Blue (EMB) and MacConkey agars. Bacteria were identified using API 20E test kit; antibiotic susceptibility test were also determined and identification of resistance gene was carried out using molecular procedures. The viable cells in soil samples ranged from 0 to 2.44 × 10¹⁰ cfu/ml. Pseudomonas luteola, Salmonella choleraesuis spp arizonae, Escherichia coli 1, Enterobacter aerogenes, etc. were the predominant isolates. Sixty-seven percent of isolates were resistant to Penicillin G while 79% were resistant to Spectinomycin. The resistance genes detected in most isolates were Van A, InuA, Sul2, bla_TEM and Otr B. The results showed that bacterial pathogens isolated from pig farm soil were not only diverse but also possessed multiple Antibiotic Resistance Gene (ARG) and this may have possible dire consequences on the environment and public health.

Key words: Microbes, pathogens, pollution, antibiotics, resistance gene, environment.