Abstract

Rapid, reliable and quantitative assays of virus universal detection are essential for fighting against Canine Parvovirus (CPV). A rapid fluorescence probe-based real-time polymerase chain reaction (RT-PCR) for detecting Canine Parvovirus was established. Primers were designed according to VP2 protein gene by Primer Premier 5.0. In response, a sensitive assay with the TaqMan probe was developed and validated. Ten-fold successive dilutions of positive CPV DNA plasmids were used to measure the sensitivity of (RT-PCR). Amplifying curve showed that this method could successfully amplify 10² copies/μL CPV gene, meanwhile reference Porcine Parvovirus (PPV), Porcine Circovirus (PCV) and blank control were all negative. The assay system showed high reproducibility with CV<3%. Thus the newly-built real-time PCR assay was indicated to be a rapid, sensitive and specific test for detecting CPV.

Key words: Canine Parvovirus, VP2 gene, real-time polymerase chain reaction.