African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Sequencing and phylogenetic analysis of Clostridium septicum alpha toxin gene from Brazilian field and vaccine strains

Ronnie Antunes de Assis1, Antonio Augusto Fonseca Júnior1, Franciele Maboni Siqueira2*, Marcelo Fernandes Camargos1, Felipe Masiero Salvarani3, Prhiscylla Sadanã Pires3, Rodrigo Otávio Silveira Silva3, Ricardo Aurélio Pinto Nascimento1, Agueda Palmira Castagna de Vargas4 and Francisco Carlos Faria Lobato3
Laboratório Nacional Agropecuário de Minas Gerais, LANAGRO-MG. Av. Rômulo Joviano s/n, CP 35/50, CEP 33600-000, Pedro Leopoldo, MG, Brasil. 2Programa de Pós-Graduação em Bioquímica, Centro de Biotecnologia- Universidade Federal do Rio Grande do Sul. Av. Bento Gonçalves, 9500, prédio 4342, setor IV. CP 15005. CEP 91501970, Porto Alegre, RS, Brasil. 3Escola de Veterinária da Universidade Federal de Minas Gerais, Depto de Medicina Veterinária Preventiva. Av. Antônio Carlos 6627, CP 567, Campus da UFMG, CEP 30123-970. Belo Horizonte, MG, Brasil. 4Universidade Federal de Santa Maria, Laboratório de Bacteriologia, Depto de Medicina Veterinária Preventiva. Centro de Ciências Rurais. Prédio 44, sala 5137, CEP 97105-900, Santa Maria, RS, Brasil
Email: [email protected]

  •  Accepted: 08 February 2012
  •  Published: 29 February 2012

Abstract

In this study, sequencing and phylogenetic analysis of partial sequences of the gene that encodes   alpha toxin from field and vaccine strains of Clostridium septicum from Brazil were performed to analyze genetic differences in  these strains. Alpha toxin nucleotide and amino acid sequences from five field strains of C. septicum and four strains used in the production of vaccines against clostridiosis were compared. Based on this sequence analysis, the C. septicum strains can be classified in two groups. Group 1 presents the majority of the sequences; including two vaccine strains and is very conserved. Group 2 presents sequences with significative number of changes in the nucleotide and amino acid sequences, and contains the other two vaccine strains used in the production of vaccines in Brazil. Therefore, genetic diversity occurs in Brazilian C. septicum strains.

 

Key words: Clostridium septicum, alpha toxin, sequencing, phylogenetic analysis.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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