Abstract

The aim of the research work is polyphyto combination compression of different antilithiogenic agent and to screen its toxicity by Caco-2-cell line. Polyphyto combination was prepared by geometrical dilution method; it was subjected for aqueous and alcoholic extract. The formulation and extract were screened for its initial in vitro toxicity study using Caco-2-cell line by 3-(4, 5 dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay method for aqueous extract of formulation. It showed nontoxic to Caco-2-cell line, even at high dose, that is, 1000 µg/ml, whereas aqueous extract (FH₁)   exhibited moderate toxicity CTC 50 value 875±35.3 µg, FH₁ crude formulation showed significant toxicity against Caco-2-cells with low concentration with CTC 50 value 382.75 based on this result the conclusion was drown that the formulation FH₂ and aqueous extract of FH₁ is devoid of toxicity. The cells were dissociated with TPVG solution (0.2% trypsin, 0.02% EDTA, 0.05% glucose in PBS).

Key words: Cytotoxicity, dimethyl sulfoxide (DMSO), flavones glycosides.