Abstract

The protective effect of Acanthopanax senticosus B (ASB) on apoptosis in Rat adrenal pheochromocytoma cell line (PC12) cell induced by sodium cyanide (NaCN)/glucose deprivation was studied. The PC12 cell was cultured in RPMI-1640 medium supplemented with 10% newborn calf serum at 37°C in a 5% CO₂ incubator. NaCN/glucose deprivation was conducted to establish an oxidative-stress injury model in vitro. The protective effect of ASB was evaluated by using this model. We compared the lactate dehydrogenase (LDH) release rate and malondialdehyde (MDA) content of PC12 cells in different treatment group. The cell apoptosis was detected by using flow cytometry (FCM) and the cell proliferation by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. These results showed that pretreatment with ASB could improve cell growth and proliferation in a concentration dependent manner, reduce the release of LDH and MDA content, and inhibit the NaCN/glucose deprivation-induced apoptosis of PC12 cells. Therefore, these results suggested that ASB possessed the protective activity of PC12 cells injury by NaCN/glucose deprivation in vitro.

Key words: Acanthopanax senticosus B, PC12 cell, sodium cyanide, glucose deprivation, protective effect.