Diabetic ulcers are hard or unable to heal or heal without the restoration of the function. Piper betle L. is known as a valuable traditional medicine to cure open wounds through its antibacterial, anti-inflammatory capabilities and the stimulation of fibroblast proliferation. In addition, adipose-derived stem cells (ADSCs) are able to renew themselves and differentiate into many cell types when repairing the wounded organs in the body. Therefore, this study indicates the therapeutic effect of ADSCs combined with the stock extraction Piper betle L. On the process of healing chronic wounds. Method: mice with chronic diabetes and created ulcers were divided randomly as 4 groups. Group 1: control (n=15), group 2: Piper betle L. extraction (n=15), group 3: injections ADSCs (n=15) and group 4: injections ADSCs combined with Piper betle L. extraction (n=15). Results: wounds in the control group, the Piper betle L. extraction group and the ADSCs group healed on the days 18, 17 and 16, respectively. Whereas, the ADSCs group combined with the Piper betle L. extraction healed on the 14th day. Conclusion: Piper betle L. extraction and ADSCs are considered as a paracrine signal to the proliferation and migration of fibroblasts and keratinocyte to heal faster. Besides, Piper betle L. also has antibacterial properties, so when combining it with ADSCs in treatment, it will speed up the healing process of diabetic wounds.

Keywords: Piper betle L., diabetes, ulcers, adipose-derived stem cells (ADSCs)