Abstract

Marine and freshwater shrimp are overexploited worldwide due to their high commercial value being usually fished in a predatory manner. The concern about conserving the stocks for economical or ecological reasons has been threatened by the absence of molecular markers for the majority of these species. In this work, we described ten anonymous microsatellites isolated from an enriched-library with magnetic beads technology of Penaeus (Litopenaeus) vannamei genome, considered the most economically important marine shrimp in the world. In addition, we tested these loci and a panel of 21 Simple Sequence Repeat from Expressed Sequence Tag (EST-SSR) loci, also isolated from P. vannamei, in ten other marine and two freshwater shrimp species. The cross species amplification success rate ranged from 32 to 77% in Macrobrachium jelskii and Penaeus (Litopenaeus) schmitti, respectively. These microsatellites appear conservative in a wide spectrum of shrimp groups, facilitating genetic studies in those species in which no microsatellites have been yet described.

Key words: Conservative, Simple Sequence Repeat (SSR), shrimp genetic, penaeid, Macrobrachium.