Poultry management generates high amounts of wastes which may result in disposal and pollution threats. It is an ecological place where there are large and diverse numbers of microorganisms. This was therefore designed to investigate bacteria diversities, their antibiotics resistance and possible implication in transporting it over long distance as manure. Dry poultry waste samples were collected from poultry waste packing sites at Idigba, Ilora, Oyo State. Bacterial cultures were carried out on nutrient and MacConkey agar using pour plate techniques. Identification of the isolates based on morphological and biochemical characteristics. Also, antibiotics susceptibility test was carried out using disk diffusion method and interpreted. The multi-drug resistant bacteria were challenged with ethyl and methanol extract of pawpaw seed. Fifty (50) bacteria were isolated and identified. The bacteria distributions belong to seven genera and as follows: Serratia fonticola (16), Klebsiella Pneumoniae (7), Lactobacillus fermenti (6), Lactobacillus delbruecki (5), Serratia liquefaciens (4), Pseudomonas spp. (3), Campylobacter spp. (2), Klebsiella oxytoca (2), Aeromonas veronii (2), Morganella morganii (1), Serratia marcescens (1), Vibrio spp. (1). Majority of the bacteria investigated were observed to have high resistance index which indicate multi-drug resistance. Although, most of the bacteria isolates were found to be susceptible to Ofloxacin and Ciprofloxacin. The resistant bacteria were found to be susceptible to ethyl and methanolic extracts of pawpaw seed which showed possibility of discovering novel antibiotics from this substance. It has been found that there are high diversities of bacteria in the poultry waste with high resistance index.

Keywords: Pawpaw seed, Novel, Multiple Antimicrobial Index, Poultry waste, Bacteria.