Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Isolation and identification of phytochemical constituents from Scrophularia takesimensis

Hye Min Kim1, Mi-Jeong Ahn2, and Sanghyun Lee1*
1Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Republic of Korea. 2College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju 660-751, Republic of Korea.
Email: [email protected]

  •  Accepted: 12 April 2012
  •  Published: 14 June 2012

Abstract

Phytochemical constituents were isolated from Scrophularia takesimensis (Scrophulariaceae) using repeated chromatography and prep-HPLC. Their structures were elucidated as stigmast-5-en-3-ol (1), α-spinasterol 3-O-β-d-glucopyranoside (2), 5-hydroxypyrrolidin-2-one (3), trans-cinnamic acid (4), 4-methoxycinnamic acid (5), 2-methoxycinnamic acid (6), and 5,7-dihydroxy-4′-methoxyflavone (7) by spectroscopic analysis. All compounds were isolated for the first time from S. takesimensis and compounds 2, 3, 6, and 7 were isolated for the first time from Scrophularia species. Among them, 5,7-dihydroxy-4′-methoxyflavone (7) exhibited strong AR inhibitory activity, with an IC50 value of 4.96 μM. The content of 5,7-dihydroxy-4′-methoxyflavone (7) was measured in aerial parts of S. takesimensis, S. kakudensis and S. boreali-koreana (54.3, 25.4, and 15.0 μg/g, respectively) by HPLC.

 

Key words: Aldose reductase inhibition, HPLC analysis, phytochemical constituent, Scrophularia takesimensis.

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