Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3832

Full Length Research Paper

Anatomical and molecular identification of “guaco” Mikania glomerata and Mikania laevigata (Asteraceae), two important medicinal species from Brazil

Carolina Lopes Bastos1*, Camila Souza da Mata1, Vitor Hugo Maia2, Rafael Augusto Xavier Borges1, Luciana Ozório Franco1, Paulo Cavalcanti Gomes Ferreira2 and Neusa Tamaio1
1Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Diretoria de Pesquisa Científica, Rua Pacheco Leão 915, Rio de Janeiro, RJ 22460-030, Brazil. 2Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil.
Email: [email protected]

  •  Accepted: 22 June 2011
  •  Published: 16 September 2011

Abstract

Mikania glomerata Sprengel and Mikania laevigata Schultz Bip. ex Baker are native Brazilian lianas, popularly known as guaco and mainly used as bronchodilators. Based on morphological and anatomical leaf features, preliminary data indicate substantial similarity between the two species, requiring further studies to increase the accuracy of their diagnosis. However, it is still unclear which of the two species is commercially sold. Therefore, in the anatomical analysis, three individuals of each species were treated according to the usual methodology for stem anatomy. Molecular analysis was performed on four specimens of M. glomerata and six of M. laevigata, using three plastid loci (matK,rbcL and trnH-psbA) and the ITS2 locus, which have been proposed as plant barcodes. Our results, including anatomical descriptions and statistically derived quantitative data, show that the anatomical structural standard is very similar between the species. Molecular data corroborate the morphological data in pointing to the total similarity between the two species observed in the loci used. Based on these results, we conclude that M. laevigataand M. glomerata could be unified in terms of nomenclature.

 

Key words: Asteraceae, liana, matKrbcLtrnH-psbA, ITS2, plant molecular biology, stem anatomy.