Abstract

Yield of polysaccharides from Lycium barbarum obtained by hot water and followed by precipitation with ethanol extraction through an orthogonal experiment were investigated to get the best extraction conditions. The results showed that the highest yield was obtained when extraction temperature, extraction time and ratio of solvent to raw material were 93°C, 4.3 h and 43, respectively. The anti-athletic fatigue activities of polysaccharides from Lycium barbarum were evaluated by the weight-loaded swimming model in mice. The results showed that polysaccharides from Lycium barbarum have noticeable anti-athletic fatigue effect on mice. It prolonged the weight-loaded swimming time, prevent the increase of blood lactic acid of mice after swimming and increase the content of hepatic glycogen of mice after swimming. The strongest effect on most biomarkers was seen with 60 mg/kg dose.

Key words: Polysaccharides from lycium barbarum, orthogonal experiment, anti-athletic fatigue, extraction.