African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6574

Short Communication

Evaluation of substrates for multiplication of bio-agent, Trichoderma viride

R. Chakrabarty
  • R. Chakrabarty
  • Department of Plant Pathology, AAU Jorhat-785 013, Assam, India.
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G. C. Acharya
  • G. C. Acharya
  • CPCRI, Research Centre, Kahikuchi Guwahati-781 017, India.
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T. C. Sarma
  • T. C. Sarma
  • Department of Botany, Gauhati University, Ghy-781 014, Assam, India.
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  •  Received: 08 October 2013
  •  Accepted: 16 June 2014
  •  Published: 20 June 2014

Abstract

An experiment was conducted using locally available substrates to mass multiply Trichoderma viride on different substrates. Experiment was conducted during June to August for 2 years (2011 and 2012). Result revealed that sporulation was observed after 5 days of incubation in cowdung, followed by all other substrates after 7 days of incubation. Maximum sporulation (51.50 × 108 cfu g-1) was observed in cowdung on 15th day, which is followed by rice bran, talc powder, rice straw, banana leaf, arecanut leaf, coconut leaf, neem cake and vermicompost with 48.12 × 108, 44.5 × 108, 39.00 × 108, 29.00 × 108, 21.4 × 108, 18.2 × 108, 15.5 × 108 and 10.45 × 108 cfu g-1, respectively. Nevertheless, up to 90 days of observation, significantly superior growth (39.1 × 108 cfu g-1) was noticed on cowdung in comparison to other substrates.

 

Key words: Trichoderma viride, substrates, mass multiplication.