African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6691

Full Length Research Paper

Initiation of callus from different genotypes of Sorghum bicolor L. Moench

Zeinab Kanani
  • Zeinab Kanani
  • Department of Botany, Faculty of Science, University of Khartoum, P. O. Box 321, Khartoum, Sudan.
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Sayadat El Tigani Mohammed
  • Sayadat El Tigani Mohammed
  • Department of Botany, Faculty of Science, University of Khartoum, P. O. Box 321, Khartoum, Sudan.
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  •  Received: 28 February 2019
  •  Accepted: 18 July 2019
  •  Published: 30 April 2020

Abstract

The study aimed to examine three genotypes of sorghum for their response in tissue culture using Murashige and Skoog (MS) medium supplemented with auxins (2,4-dichlorophenoxy acetic acid (2,4-D), naphthalene acetic acid (NAA)) and cytokinins (Kinetin, 6-benzyle amino purine (BAP)) at different concentrations. The cultures were initiated from different explants (seed, embryo and hypocotyl). The response of explants varied with the genotype. Callus culture were initiated successfully on MS medium supplemented with 2 mg/l 2,4-D and 0.5 mg/l Kinetin from G4 (L58) seedling and embryo explants. G2 (94) hypocotyl explants gave callus on media supplemented with 2,4-D only. G5 (2) explants failed to initiate callus but bulging from the embryo explants was observed on MS medium supplemented with 2 mg/l 2,4-D and 0.5 mg/l Kinetin. The initiated calluses differed morphologically and in their growth rates.

Key words: Sorghum bicolor, callus formation, embryo, Kinetin, 2,4-dichlorophenoxy acetic acid (2,4-D).