African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6860

Full Length Research Paper

Characterization of Uromyces appendiculatus isolates collected from snap bean growing areas in Kenya

  E. E. Arunga1*, J. O. Ochuodho2, M. G. Kinyua1 and J. O. Owuoche3        
  1Department of Biotechnology, Chepkoilel University College, Box 1125-30100 Eldoret, Kenya. 2Department of Seed, Crops and Horticultural Sciences, Chepkoilel University College, Box 1125-30100 Eldoret, Kenya. 3Department of Crops, Horticultural and Soil Science, Egerton University, Box 536, Egerton, Kenya.
Email: [email protected]

  •  Accepted: 08 October 2012
  •  Published: 30 November 2012

Abstract

 

Bean rust (Uromyces appendiculatus (Pers.:Pers.) Unger var. appendiculatus) is one of the most devastating and variable pathogens of common bean (Phaseolus vulgaris L.) worldwide that can cause total crop loss. Characterization of bean rust races can help in screening resistant materials during gene pyramiding. The aims of the present research were first, to characterize bean rust isolates collected from snap bean growing areas in Kenya. Secondly, to identify which of the available rust resistance genes in common bean differentials are most effective to control rust in those areas. Snap bean leaf tissues with rust pustules were collected from different farms in eight locations in central and western Kenya during the years 2010 and 2011. Forty seven single pustule isolates were obtained and inoculated on 12 bean rust differential cultivars. For consistent results, the inoculation was repeated twice. The new international classification system and the binary nomenclature grouped the 47 single pustule isolates of U. appendiculatus into 9 different races, most of this affected the Andean gene pool. Hierarchical cluster analysis grouped the races into two major clusters depending on the virulence of the races on the host differential cultivars. The most resistant genes for pyramiding in Kenya were identified as Ur-5, Ur-11 and Ur-CNC. An important output of this study was the identification of races with potential use during gene pyramiding process.

 

Key words: Uromyces appendiculatus, snap bean, races, genes, characterization.