Abstract

An interlocational study was carried out to assess the effect of different agro-climatic locations on the secondary metabolite production in the medicinal plant Andrographis paniculata. Three accessions of A. paniculata were grown in three different locations of India differing in climatic conditions in RBD experimental plots. The andrographolide content in the plant samples of three accessions of A. paniculata was estimated from plants collected from these three locations, namely Anand, Kalyani and Solan. The three accessions with no morphological differences were characterised using RAPD and ISSR profiles. As the plants were grown in different agro-climatic conditions, as expected, there was variation in the andrographolide content in the three accessions. Samples collected from Kalyani were found to contain maximum andrographolide content as compared to the other two locations.

Key words: Andrographispaniculata, andrographolide, locations, high-performance liquid chromatography (HPLC), random amplified polymorphic DNA (RAPD).