African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Bacterial pathogens of intramammary infections in Azeri buffaloes of Iran and their antibiogram

Reza, Vajdi Hokmabad1*, Mehran, Farhoudi Mogaddam2, Majid, Mohammad Sadegh3 and Hamid, Mirzaii4
1Department of Clinical Science, Faculty of Veterinary Specialized Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2Department of clinical science, Veterinary Medicine Faculty, Karaj Branch, Islamic Azad University, Karaj, Iran. 3Department of Clinical Science, Veterinary Medicine Faculty, Garmsar Branch, Islamic Azad University, Garmsar, Iran. 4Department of Food Hygiene, Veterinary Medicine Faculty, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Email: [email protected] or [email protected]

  •  Accepted: 12 May 2010
  •  Published: 04 June 2011

Abstract

The aim of this study was to determine the bacterial causes, their antibiogram and prevalence of intramammary infection (IMI) in the buffaloes and quarters of Azeri ecotype in Tabriz, Iran and assess any relationships between IMI and somatic cell count (SCC). After conducting California Mastitis Test (CMT) in farms, quarter- based milk samples were collected from 300 buffaloes. Also 150 samples were collected for bacterial culture and count. Based on CMT, 13.87% of quarters and 23.66% of buffaloes had subclinical mastitis, the sensitivity and specificity of CMT for infections with all bacteria and infections with major pathogens were 55 and 67.39, 70 and 50% respectively. Coagulase-negative staphylococci (CNSs) were the most common pathogens. Isolated bacteria had no significant effects on mean SCC of infected quarters. The most effective antibiotics against all isolated agents were Cephtiofur and Trimetoprim+ Sulfametoxazol with a sensitivity rate of 97.3and 94.6% respectively. The SCC and total bacterial count (TBC) of infected quarters were significantly higher than healthy ones (p< 0.05). Based on the results of current study, CMT has acceptable sensitivity and specificity in diagnosis. Coliforms are not probably very important in buffaloes intramammary infections.

 

Key words: Buffalo, mastitis, intramammary infection, SCC, bacterial culture.