African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Biological control of the bacterial wilt Ralstonia solanacearum by bioprotector with fungi chitosan from Cunninghamella elegans on tomatoes

Wagner Silva Oliveira
  • Wagner Silva Oliveira
  • Department of Agronomy, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n, 52171-900, Recife, Pernambuco, Brazil.
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Iwanne Lima Coelho
  • Iwanne Lima Coelho
  • Department of Agronomy, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n, 52171-900, Recife, Pernambuco, Brazil.
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Jessica Rafaella Sousa Oliveira
  • Jessica Rafaella Sousa Oliveira
  • Department of Agronomy, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n, 52171-900, Recife, Pernambuco, Brazil.
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Maria Camila Barros Silva Leite
  • Maria Camila Barros Silva Leite
  • Department of Agronomy, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n, 52171-900, Recife, Pernambuco, Brazil.
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Thatiana Montenegro Stamford Arnaud
  • Thatiana Montenegro Stamford Arnaud
  • Department of Agronomy, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n, 52171-900, Recife, Pernambuco, Brazil.
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Newton Pereira Stamford
  • Newton Pereira Stamford
  • Department of Agronomy, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n, 52171-900, Recife, Pernambuco, Brazil.
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Emmanuella Vila Nova Silva
  • Emmanuella Vila Nova Silva
  • Department of Agronomy, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n, 52171-900, Recife, Pernambuco, Brazil.
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  •  Received: 29 September 2016
  •  Accepted: 27 October 2016
  •  Published: 05 January 2017

Abstract

Fertilization is one of the most important means to improve plant production and nutrient uptake. Tomatoes plants are very exigent on fertilizers and sensitive to diseases. For satisfactory yield and fruit quality soil fertility and diseases need to be controlled. The aim of this paper was to evaluate the effectiveness of a bioprotector that contains fungi chitosan as an alternative biofertilizer, which releases nutrients and induces resistance against tomatoes wilt by Ralstonia solanacearum bacteria. The treatments were: 1- soluble NPK fertilizers (NPKF) in recommended rate (RR), 2- bioprotector (NPKP) with fungi chitosan in half of recommended rate (50% RR), 3– NPKP 100% RR, and 4– NPKP 150% RR. Treatments without bacterial inoculation (PO) and with bacterial inoculation (P1) were added to evaluate the plant characteristics (plant height, shoot diameter, number of leaves in ramification, fresh and dry matter of shoots). The symptom classes used to observe the induction of resistance were: (--) plants with no disease symptoms; (-) plant with slight symptoms; (+) plants with drastic symptoms and (++) plants died. Plants receiving the soluble fertilizers (NPKF) showed drastic disease symptoms one week after R. solanacearum inoculation, and all the plants died two weeks after inoculation. Plants with NPKP that contains Cunninghamella elegans in rates 50, 100 and 150% RR induced resistance for bacterial disease and promote better plant characteristics. The results showed that the bioprotector displays normal characteristics. The protector may be used as alternative for conventional fertilizers, especially inducing resistance for bacterial control.

 

Key words: Lycopersicon lycopersicum, Ralstonia solanacearum, biopolymers, organic bioprotector, phytopatogenic bacteria, resistance induction.