African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Virulence of entomopathogenic fungi to adults and eggs of Nilaparvata lugens Stal (Homopera: Delphacidae)

Maoye Li1, Huafeng Lin1*, Shiguang Li1, Peirong Chen2, Li Jin1 and Jun Yang1
1School of Plant Protection, Anhui Agricultural University, Hefei, Anhui 230036, China. 2School of Science, Anhui Agricultural University, Hefei, Anhui 230036, China.
Email: [email protected]

  •  Accepted: 21 February 2012
  •  Published: 30 April 2012

Abstract

Brown plant hopper (BPH), Nilaparvata lugens is a devastating insect pest of rice. Effective control measures are desperately needed. Entomopathogenic fungi, such as Beauveria and Metarhizium, have shown great potential for the management of some sucking pests. In this study, to explore alternative strategy for sustainable control of the pest population, 12 isolates of Beauveria and Metarhizium from sucking pests were bioassayed under the concentrated standard spray of 1 × 108 conidia/ml in laboratory. The cumulative mortalities of adult ranged from 17.2 to 82.1%, 10 days after inoculation. The virulence among all tested isolates exhibited significant differences. The two most virulent isolates, M. flavoviride (Mf82) and M. anisopliae (Ma20) with cumulative mortalities of 82.1 and 65.4%, respectively, were selected to detect the virulence to gravid female, female and male of BPH with suspension of 1 × 108 conidia/ml. The susceptible sequence was gravid female > female > male. Isolates of Beauveria and Metarhizium could infect eggs of BPH. The infected eggs shrunk in shape, then turned brown and eventually had outgrowths of the sprayed fungus when maintained under high moist conditions. The virulence of Mf82 to the eggs was the greatest, whose cumulative mortality was 60.8%, 10 days after inoculation. The most virulent isolate selected in this study, M. flavoviride (Mf82) is a promising candidate for microbial control of BPH.

 

Key words: Nilaparvata lugens, BeauveriaMetarhizium, virulence, biocontrol agents, eggs.