African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Influence of some culture media on antifungal activity of Pseudomonas fluorescens UTPF61 against the Sclerotinia sclerotiorum

  Fereshteh Heidari-Tajabadi1*, Masoud Ahmadzadeh1, Asma Moinzadeh2 and Maryam Khezri1  
  1Laboratory of Biological Control of Plant Disease, Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Iran. 2Department of Agronomy and Plant Breeding, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.  
Email: [email protected]

  •  Accepted: 25 October 2011
  •  Published: 12 December 2011



Fluorescent pseudomonads are ubiquitous bacteria that are common inhabitants of the rhizosphere and are the most studied group within the genus Pseudomonas. Strain UTPF61 is a biocontrol agent againstSclerotinia wilt of sunflower, an important disease of sunflower caused by Sclerotinia sclerotiorum. Thisbacterium was selected out of 47 strains. It was grown in eight culture media containing different carbon sources, such as sucrose, glucose, corn steep liquor, fructose, glycerol, sugar beet molasses, manitol and starch. Our results showed that the starch has a considerable effect (P<0.01) on the rate of bacterial growth as compared to the other carbon sources used. Investigation of antifungal activity in vitro indicated that some media containing glucose and sugar beet molasses were the most effective (P<0.01) on antagonistic efficiency, whereas the medium containing sugar beet molasses was the most effective on antagonistic efficiency in greenhouse trials (with 87% healthy plants).


Key words: Antagonistic efficiency, antifungal activity, biological control, carbon sources, growth rate.