African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6574

Full Length Research Paper

Establishment of a highly efficient in vitro regeneration system for Chinese kale (Brassica oleracea L. var. alboglabra)

Yaoguo Qin1, Jianjun Lei2, Bihao Cao2, Guoju Chen2, Yongli Zhai3 and Cuiqin Yang3*      
1College of Horticulture, Sichuan Agricultural University, Ya'an 625014, China. 2College of Horticulture, South China Agricultural University, Guangzhou 510642, China. 3College of Agriculture, Sichuan Agricultural University, Ya'an 625014, China.  
Email: [email protected]

  •  Accepted: 17 July 2012
  •  Published: 07 August 2012

Abstract

The effects of different combinations and concentrations of plant growth regulators on differentiation frequency were studied using hypocotyls, cotyledons, and flamingo-bills as explants to establish an effective in vitroregeneration system for Chinese kale. The results showed that hypocotyls on a culture medium consisting of Murashige and Skoog (MS) medium + 1% sucrose + 2.0 mg/L BAP + 7.0 mg/L AgNO3 achieved a differentiation frequency of 86.7%. The differentiation frequency of cotyledons on MS + 3% sucrose + 2.0 mg/L BAP + 0.40 mg/L NAA + 5.0 mg/L AgNO3 was 94.0%. Flamingo-bills produced shoots on MS + 3% sucrose + 2 mg/L BAP, and the average number of shoots obtained per explant was 4.5. Adventitious shoots produced roots when inoculated on MS + 0.2 mg/L NAA.

 

Key words: Chinese kale, regeneration, differentiation, hypocotyl, cotyledon, flamingo-bill.