The effects of different combinations and concentrations of plant growth regulators on differentiation frequency were studied using hypocotyls, cotyledons, and flamingo-bills as explants to establish an effective in vitroregeneration system for Chinese kale. The results showed that hypocotyls on a culture medium consisting of Murashige and Skoog (MS) medium + 1% sucrose + 2.0 mg/L BAP + 7.0 mg/L AgNO3 achieved a differentiation frequency of 86.7%. The differentiation frequency of cotyledons on MS + 3% sucrose + 2.0 mg/L BAP + 0.40 mg/L NAA + 5.0 mg/L AgNO3 was 94.0%. Flamingo-bills produced shoots on MS + 3% sucrose + 2 mg/L BAP, and the average number of shoots obtained per explant was 4.5. Adventitious shoots produced roots when inoculated on MS + 0.2 mg/L NAA.
Key words: Chinese kale, regeneration, differentiation, hypocotyl, cotyledon, flamingo-bill.
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