African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6574

Full Length Research Paper

In vitro evaluation of antitrypanosomal and cytotoxic activities of soil actinobacteria isolated from Malaysian forest

H. Lili-Sahira
  • H. Lili-Sahira
  • Natural Product Division, Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia.
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K. Getha
  • K. Getha
  • Natural Product Division, Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia.
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A. Mohd Ilham
  • A. Mohd Ilham
  • Malaysian Institute of Pharmaceutical and Nutraceutical, Bukit Gambir 11700, Penang, Malaysia.
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I. Norhayati
  • I. Norhayati
  • Natural Product Division, Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia.
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M. M. Siti-Syarifah
  • M. M. Siti-Syarifah
  • Natural Product Division, Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia
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A. Muhd Syamil
  • A. Muhd Syamil
  • Natural Product Division, Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia
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J. Muhd Haffiz
  • J. Muhd Haffiz
  • Natural Product Division, Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia
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G. Hema-Thopla
  • G. Hema-Thopla
  • Natural Product Division, Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia
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  •  Accepted: 28 January 2013
  •  Published: 15 February 2013

Abstract

 

A total of 83 actinobacteria isolates were successfully revived from cryovials stored in -80°C FRIM actinobacteria culture collection (FACC). Based on macromorphological observation, Streptomyces-like group is predominant among all the isolates. The representative actinobacteria isolates were grown in three different fermentation media (M1, M2 and M3) and a total of 249 culture broth extracts obtained were evaluated for in vitro antitrypanosomal activity against Trypanosoma brucei brucei strain BS221. Five extracts (2.0%) exhibited strong activity (score 3) with an IC50 value ≤ 1.56 µg ml-1 and 34 extracts (13.7%) exhibited moderate activity (score 2) of 1.56 µg ml-1 < IC50 ≤ 12.5 µg ml-1 . Extracts showing score 3 and score 2 activities were further tested for cytotoxicity. Eight extracts exhibited good selectivity with a SI (Selectivity Index) value ≥ 20. Among the isolates showing good selectivity, isolate FACC-A032 that belonged to the genus Streptomyces produced extract with the highest antitrypanosomal activity (IC50 = 0.23 µg ml-1) and high selectivity (SI = 76.417). Growth profile study of isolates FACC-A032 in medium M3 exhibited maximum antitrypanosomal activity at day eight of fermentation with IC50 = 0.15 µg ml-1 and SI = 154.75. This is the first study of in vitroantitrypanosomal activity of soil actinobacteria isolated from Malaysian forest.

 

Key words: Antitrypanosomal, trypanosomiasis, actinobacteria, Alamar blue assay, Streptomyces.