African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6848

Full Length Research Paper

Genetic diversity of Cleome gynandra germplasm in Burkina Faso using SSRs molecular markers

SAKANDE Boureima
  • SAKANDE Boureima
  • Laboratoire Biosciences, Unité de Formation et de Recherche en Sciences de la Vie et de la Terre,Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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KIEBRE Zakaria
  • KIEBRE Zakaria
  • Laboratoire Biosciences, Unité de Formation et de Recherche en Sciences de la Vie et de la Terre,Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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BATIENO Teyiouê Benoît Joseph
  • BATIENO Teyiouê Benoît Joseph
  • Centre National de la Recherche et Technologique, Institut de l’Environnement et de Recherches Agricoles, Kamboinsé (INERA / Kamboinsé), Burkina Faso.
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SAWADOGO Pingawindé
  • SAWADOGO Pingawindé
  • Centre Universitaire de Tenkodogo, Burkina Faso.
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BATIONO/KANDO Pauline
  • BATIONO/KANDO Pauline
  • Laboratoire Biosciences, Unité de Formation et de Recherche en Sciences de la Vie et de la Terre,Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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  •  Received: 21 January 2023
  •  Accepted: 18 August 2023
  •  Published: 31 August 2023

Abstract

Cleome gynandra L. is an important traditional leafy vegetable in Burkina Faso diets. Because of its high nutritional value, it makes a good dietary supplement, providing an important nutritional and medicinal requirement. The aim of the present study was to assess the genetic diversity and intra- and interpopulation relationships of the C. gynandra collection using SSR molecular markers specific to C. gynandra. Nine microsatellite primers were used to assess the genetic diversity of 33 C. gynandra accessions. A total of 50 alleles were counted (an average of 5.56 alleles per marker). Polymorphism information content (PIC) ranged from 0.82 to 0.43 for markers CG01 and CG033, respectively, with an average of 0.60, showing the high genetic diversity of the C. gynandra collection studied. In fact, six markers are highly informative (PIC > 0.5). Inter-morphotype differentiation showed an STF of 0.17 was recorded between the green morphotype and the dark purple morphotype. In effect, the two morphotypes are different. The organization and structuring of diversity resulted in three distinct genetic groups, irrespective of morphotype color, and accession origin.

Key words: Molecular markers, SSRs, Cleome gynandra, Burkina Faso.