This experiment was conducted in Ardestan University in an experiment based on randomized complete block design with four replications. Each replication consisted of 12 treatments in four fertilizer levels including 2.5 per thousand of Fe-sulfate solution, 2.5 per thousand of Zn-sulfate solution in three stages of growth (4, 8 and 12 leaves). During the experiment, the height of plant, number of stem branches, follicle number in plant, seed number in follicle, 1000- grain weight, biologic yield, seed yield/plant and harvest index were assessed. This study showed that using spraying had significant differences in different growth stages except in the factors like plant height, times of flowering and weight of thousands grain. Also, using all microelement treatments had significant effects to the level of 1%. The most grain yield was in eight-leaf-stage with 172.35 kg/ha and after that four-and twelve-leaf-stages were in the second level. In case of using spraying treatments, the best results were related to 2.5 per thousand of Fe-sulfate solution and the least were related to control and 2.5 per thousand of Zn-sulfate solution. In evaluating the interaction between treatments, the best yield was related to 2.5 per thousand of Fe-sulfate solution in eight- leaf- stage and the least related to 2.5 per thousand of Zn-sulfate solution and control. In the end, spraying of 2.5 per thousand of Fe-sulfate solution in eight- leaf- stage without using Zn-sulfate, in many cases dedicated the best results to itself. This formulation can be used for increasing yield, enhancing the products and removing food deficiencies.
Key words: Nigella sativa L., Fe-sulfate, Zn sulfate, seed yield and growth stage, spraying.
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