A whole-plant open-system chamber was developed to measure CO2 exchange in Phalaenopsis amabilis. The consistent and continuous curves of CO2 gas exchange rhythms at various conditions, sampled at 1-min interval, were used to evaluate the factors affecting on the CO2 exchange pattern. The influencing factors included the day/night temperature, sample size, light period, light intensity and water stress. The adequate growth conditions of P. amabilis were 26 to 30°C day temperature, 20 to 22°C night temperature, 14 to 16 h light and 180 to 215 μmol m-2s-1 of light intensity. Water stress had the most significant effect on the CO2 exchange pattern. The gas exchange measurement system developed in this study is a useful and reliable measurement system for studying the physiological characteristics of Phalaenopsis varieties.
Key words: CO2 pattern, open-system, Phalaenopsis, CAM plant, water stress, light rhythms.
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